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KMID : 0357419940240010027
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Korean journal of Virology
1994 Volume.24 No. 1 p.27 ~ p.34
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Expression of Bovine Viral Diarrhea Viral Envelope Protein in Prokaryotic Vector
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Abstract
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The gene corresponding envelope(E2, C-terminal of E1 and E2) of bovine viral diarrhea virus (BVDV) was expressed in downstream from the malE gene of E. coli, resulting in the expression of fusion protein with maltose-binding protein(MBP).
The expressed MBP-E2 fusion protein was affinity purified for the production of antiserum and the expression of E2 was identified by immunoblotting with antiserum against MBP-E2 fusion protein. Although the protein band with molecular weight
above
88
kDa was identified, the cell expressing C-terminal of E1 and E2 showed the degradation of expressed fusion protein. However, low molecular weight protein bands including MBP were frequently detected in immunoblotting assay, thus, indicating that
C-terminal of E1 can play a role of signal peptide after translation in similar pattern of eukaryotic cell.
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KEYWORD
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